tousimis® TEM Laboratory Supplies

# 1008A FORMALDEHYDE 20%

No preservatives. FW 30.03 HCHO, C(39.99%), O(53.29%), H(6.73%), pH 7-7.35. Merck Index 11, 4150. Ready-to-use® in your research laboratory as a primary or complimentary fixative of biological tissue-samples for electron, light microscopy, or other laboratory applications.
$158.00 (10x10ml vials)

# 1008A-5 FORMALDEHYDE 20%

No preservatives. FW 30.03 HCHO, C(39.99%), O(53.29%), H(6.73%), pH 7-7.35. Merck Index 11, 4150. Ready-to-use® in your research laboratory as a primary or complimentary fixative of biological tissue-samples for electron, light microscopy, or other laboratory applications.
$690.00 (50x10ml vials)

# 1008B FORMALDEHYDE 20%

No preservatives. FW 30.03 HCHO, C(39.99%), O(53.29%), H(6.73%), pH 7-7.35. Merck Index 11, 4150. Ready-to-use® in your research laboratory as a primary or complimentary fixative of biological tissue-samples for electron, light microscopy, or other laboratory applications.
$140.00 (100ml bottle)

# 1008B-10 FORMALDEHYDE 20%

No preservatives. FW 30.03 HCHO, C(39.99%), O(53.29%), H(6.73%), pH 7-7.35. Merck Index 11, 4150. Ready-to-use® in your research laboratory as a primary or complimentary fixative of biological tissue-samples for electron, light microscopy, or other laboratory applications.
Each bottle contains 100ml.
$1,070.00 (10x100ml bottles)

# 1008C FORMALDEHYDE, 10%

TEM grade, HCHO ultrapure, ready-to-use® Packaged under dry-microfiltered nitrogen.
$158.00 (10x10ml vials)

# 1008D FORMALDEHYDE, 10%

HCHO ultrapure, TEM grade, ready-to-use® Packaged under dry-microfiltered nitrogen (See #1008A).
$135.00 (100ml bottle)

# 1011A TOUSIMIS® FIXATIVE, for fast penetration of tissues

TEM-SEM 1.5% Glutaraldehyde/1.0% Formaldehyde in Sorensen's Buffer at pH 7.35 isotonic. A fast-penetrating and excellent fixative for biopsies and/or experimental tissue samples for TEM, LM, and SEM. The Tousimis-Johnson TEM-FIX is very popular in mailing fixed samples to the pathology or the TEM/SEM laboratory for further processing.
$158.00 (10x10ml vials)

# 1011A-5 TOUSIMIS® FIXATIVE, for fast penetration of tissues

TEM-SEM 1.5% Glutaraldehyde/1.0% Formaldehyde in Sorensen's Buffer at pH 7.35 isotonic. A fast-penetrating and excellent fixative for biopsies and/or experimental tissue samples for TEM, LM, and SEM. The Tousimis-Johnson TEM-FIX is very popular in mailing fixed samples to the pathology or the TEM/SEM laboratory for further processing.
$690.00 (50x10ml vials)

# 1012 TEMFIX™, tousimis® Special 0.625% Glutaraldehyde

0.625% tousimis® ultrapure glutaraldehyde in 99.35% isotonic phosphate sodium buffer, pH 7.35.
Sterilized. Sealed Under Filtered Dry Nitrogen.
Each bottle contains 100ml.
$925.00 (10x100ml bottles)

# 1015 TEMFIX™, tousimis® Special 1.5% Glutaraldehyde

1.5% tousimis® ultrapure glutaraldehyde in isotonic phosphate sodium buffer, pH 7.35.
Sterilized. Sealed Under Filtered Dry Nitrogen.
Each bottle contains 100ml.
$925.00 (10x100ml bottles)

# 1051 TEM/SEM Glutaraldehyde, 2.5% in Sodium Phosphate Buffer

in tousimis® sodium phosphate buffer, pH 7.35. 0.1 M. Microfiltered.
Sealed Under Dry Filtered Nitrogen.
Keep Refrigerated.
$148.00 (10x10ml vials)

# 1054 TEM/SEM Glutaraldehyde, 2.5% in 0.1M Na/K Sorensen's Phosphate Buffer

Isosmolar Na-K Phosphate Buffer, 0.1M, pH 7.3, ready-to-use®. Contains potassium dihydrogen phosphate (KH2PO4) and sodium phosphate (Na2HPO4). Used to make formaldehyde, glutaraldehyde, or osmium tetroxide fixatives. Also used as a wash buffer after fixation and before dehydration.
$148.00 (10x10ml vials)

# 1054-5 TEM/SEM Glutaraldehyde, 2.5% in 0.1M Na/K Sorensen's Phosphate Buffer

Isosmolar Na-K Phosphate Buffer, 0.1M, pH 7.3, ready-to-use®. Contains potassium dihydrogen phosphate (KH2PO4) and sodium phosphate (Na2HPO4). Used to make formaldehyde, glutaraldehyde, or osmium tetroxide fixatives. Also used as a wash buffer after fixation and before dehydration.
$665.00 (50x10ml vials)

# 3076 BDMA, for embedding

(Benzyldimethyl Amine), distilled and of highest purity 99+%, an amine accelerator for polymerization of epoxy resins. Also known as N,N-dimethyl-benzylamine, C6H5CH2N(CH3)2, FW 135.21; MP -75°C; BP 183-4°C; FP 54°C. CORROSIVE and TOXIC.
$45.00 (50ml bottle)

# 3093 DMAE, for embedding

N,N-Dimethylaminoethanol or N,N-Dimethylethanolamine, 99+% distilled, an amino accelerator for low viscosity epoxies. HO(CH2)2N(CH3)2, FW 89.14, Merck Index 11, 2834. LACHRYMATOR, CORROSIVE. Packaged under dry-microfiltered nitrogen.
$40.00 (25ml bottle)

# 3100 DMP-10, for embedding

Dimethylamino Methyl Phenol) distilled, an accelerator. May be used when a slow polymerization is desired to allow maximum epoxy infiltration into tissue specimens. C6H4OHCH2N(CH3)2, FW 151. Packaged under dry-microfiltered nitrogen.
$42.00 (25ml bottle)

# 3103 DMP-30, for embedding

2,4,6-Tri(Dimethylamino Methyl)Phenol] a distilled amine accelerator for epoxy resins. DMP-30 is a faster curing agent than DMP-10, giving a shorter time for the epoxy/anhydrite mixture to infiltrate the tissue. Better infiltration is achieved using DMP-10. Both accelerators have been successfully used with the araldites (502, 506, 6005), tEPON-812 and Thiokol LP-3. Packaged under dry-microfiltered nitrogen.
$38.00 (25ml bottle)

# 3111 DER-736, for embedding

DOW epoxy resin for low viscosity embedding, some erroneously call it the "SPURR" epoxy. First used by Kushida (1967) with Epon-812 and with ERL-4206 (vinyl cyclohexen dioxide, see tousimis® #3199).
$44.00 (100ml bottle)

# 3123 DDSA, for embedding

2-Dodecenylsuccinic Anhydride) Specially distilled for TEM sample embedding. The choice cross linking agent for epoxy resins ever since its introduction by Gavert-Glavert (1958) for the araldites; it has become the basic component of mixture A (tEPON 812 and DDSA) along with another anhydrite (NMA) component of mixture B with tEPON-812 since Luft's introduction in 1961.
$54.00 (100ml bottle)

# 3131 tEPON 812 EPOXY RESIN, a substitute for shell tEPON 812 of the default shell EPON

Substitute for Shell-Epon 812 with the same applicability. Manufactured to our specifications for TEM application. Can be used in combination with other epoxy resins (araldite M, 502, 506, 6005, DER-736, 732) and anhydrites NMA and DDSA. Contains less than 0.005% by weight of epichlorohydrin. See article by Kushida Todai J. Exp. Clin. Med. 10, 557 1985 and Luft J.B.B. Cytol. 9, 409 1961 for more information on usage. BEST BUY!
$42.00 (100ml bottle)

# 3132 tEPON 812 EMBEDDING KIT, for embedding

Introduced by tousimis® in 1976, contains: 2 x 100ml tEPON-812 Resin, 100ml DDSA (dodecenylsuccinic anhydride), 100g NMA (Nadic Methyl anhydride), 25ml DMP-30 [Tri(dimethylamino methyl)phenol], and embedding instructions.
$242.00

# 3143 NMA, for embedding

(Nadic Methyl Anhydride, Methyl-5-norbornene-2,3-dicarboxylic anhydrite) 99+% min. purity. FW 178.19, d 1.232, FP 110°C. Each lot is analyzed for percent anhydrite. A cross linking agent for epoxy resins. CORROSIVE, MOISTURE SENSITIVE. Packaged under dry-microfiltered nitrogen.
$78.00 (100g bottle)

# 3152 NSA, for embedding

(Nonenylsuccinic Anhydride), distilled for use with DER739/VCD, a low viscosity embedding of tissue for TEM. Other synonyms: Tripropenyl Succinic Anhydrite of the chemical family: allenyl succinc anhydrite (C9H17-H(C2HO)2O. Almost indefinite shelf life if properly stored. Critical component of low viscosity embedding. HYGROSCOPIC. Packaged under dry-microfiltered nitrogen.
$142.00 (100ml bottle)